More importantly, hPDLSCs can be harvested from medical waste materials such as discarded extracted teeth without additional surgery that may cause patients to experience physical deformity, pain, and considerable expense. Human PDLSCs (hPDLSCs) have proven stem cell characteristics, including osteogenic-differentiating and immune-modulating properties. Due to this multipotent differentiation ability, PDLSCs can be used in regenerative medicine because they provide a source of cells not only for dental tissue regeneration but also for repair of nondental structures such as bone and nerves. As one of the MSCs from dental origin, periodontal ligament stem cells (PDLSCs) have been isolated and tested for their ability to develop into various types of tissues in in vitro and in vivo studies. Mesenchymal stem cells (MSCs), as multipotent progenitor cells, can be isolated from adult bone marrow or prenatal tissues. Tissue engineering mimics the natural healing process to reconstruct or regenerate damaged tissue using three fundamental tools: progenitor cells, signaling molecules, and scaffolds. Therefore, Langer in 1993 proposed tissue engineering as a possible technique for periodontal tissue regeneration. While periodontal treatments such as scaling, surgical cleaning, bone grafts, and guided tissue regeneration are generally successful, the ability to regenerate the damaged tissues predictably still remains a major unmet objective for conventional treatment strategies. At present, periodontal disease treatments focus on promoting periodontal tissue regeneration by controlling inflammation and formatting new attachment. Periodontal disease involves the deterioration of periodontal bone and is the primary cause of tooth loss in adults. These results suggested that the core-shell PEI/pBMP2-PLGA scaffold fabricated by coaxial electrospinning had a good gene release behavior and showed a prolonged expression time with a high transfection efficiency. Moreover, the core-shell electrospun scaffold showed BMP2 expression for a much longer time (more than 28 days) compared with the single axial electrospun scaffold, as evaluated by qRT-PCR and western blot after culturing with hPDLSCs. ![]() At the same time, PEI/pBMP2 showed high transfection efficiency. Our results showed that pBMP2 was released at high levels in the first few days, with a continuous release behavior in the next 28 days. Then the gene release behavior was analyzed. First, the scaffold characterization and mechanical properties were evaluated. The pBMP2 was encapsulated in the PEI phase as a core and PLGA was employed to control pBMP2 release as a shell. In this study, the bioactive PEI (polyethylenimine)/pBMP2- (bone morphogenetic protein-2 plasmid-) PLGA (poly(D, L-lactic-co-glycolic acid)) core-shell scaffolds were prepared using coaxial electrospinning for a controlled gene delivery to hPDLSCs (human periodontal ligament stem cells). Scaffolds loaded with osteogenic factors improve the therapeutic effect. All rights reserved.Bone tissue engineering is the most promising technology for enhancing bone regeneration. The microreactor demonstrates excellent catalytic activity at a shorter residence time for Knoevenagel condensation reaction of benzaldehyde and ethyl cyanoacetate. ![]() The microspheres as catalysts could be easily filled into a capillary microreactor with the help of an external magnetic field. The procedure involves first pre-treating the magnetic cores with an anionic polyelectrolyte to alter the surface charge of the particles and adsorb Zn2+ cations to initiate nucleation and then growing a thin layer of ZIF-8 to form a highly reactive, magnetic core-shell microsphere The characterization by various techniques indicates that ZIF-8 shell is continuous and has an average thickness of around 100 nm. 398-404Ī facile synthesis strategy for preparing magnetic core-shell microspheres has been successfully developed. Please use this identifier to cite or link to this item: Synthesis of magnetic core-shell microspheres and their potential application in a capillary microreactor Bibliographic Details AuthorĬhemical Engineering Journal, v.
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